Abstract

Grey partridge is the native bird of Pakistan. It is present in Mianwali which is the least developed area. It is a game bird so it is a significant source of income for the area, but no data is available about its population in Mianwali. So, the purpose of this work is to study the cytochrome b gene of grey partridge to find out genetic variation in the Cytb gene of grey partridge and to gain information about the genetic diversity of this species. We collected blood samples of 8 individuals from 5 different sampling sites in the Mianwali. The length of DNA was 660 B.P. Neighbor joining, Minimum Evolution and UPGMA trees showed that there were a total of 1157 positions in the final dataset. The estimates of evolutionary divergence between Sequences results showed that a significant difference is present between birds of the same group and also among the groups. 99 % similarity Index through BLAST showed that the species that is present in this area is Francolinus Pondicerianus. Overall mean distance was found to be 2.76. Grey partridge shows more deviation. Average codons are found to be 302 in Codon usage bias.