Abstract

Black Australorp chicken is an Australian breed and now these are present all over the world. BA is attractive birds that are docile, hardy, and productive layers, making them perfect for beginners to captivity. The present study was conducted to check the effect of ascorbic acid on sperm Motility, sperm cytoplasmic membrane integrity, sperm livability and acrosome integrity of Black Australorp at different concentrations of ascorbic acid. Bird sperm is much more susceptible to cryo-damage due to its specific shape and physical properties, which lowers the survival of bird sperm. With low birth rates lines of chicken, glycerol is less harmful and most efficient cryoprotectant. Glycerol has also been shown to react with sperms metabolism, modify lipids bilayers, & lead to limited fertilizing capacity through influencing semen acrosome reaction. Semen was cooled from post-dilution stage (37ºC) to post-cooling (20ºC). Then it further cooled gradually to get post-equilibration stage (4ºC) within 24 hours. After that 7% glycerol was added to the sample. Then it was transferred to liquid nitrogen (LN₂) cylinder for 24 hours. The better results were seen at 0.5 mM treatment of ascorbic acid. Sperm showed significantly different (P < 0.05) percentage on 0.5 mM treatment on all the stages of cryopreservation rather than other treatments.